The NIH Center for Regenerative Medicine

NIH CRM Research Tools

Available Cell Lines

iPSC Stem Cell Lines

CONTROL LINES

All control lines are now available.

Note: The following cell line names have been changed, as outlined below. Please use the new names when requesting.
1. NL cell lines 1-6 from Lonza have been renamed: NL1 (old format) = NCRM-1 (new format).
2. CY1 has been renamed to CY2.

Name or Designation Description iPSC Reprogramming Method Starting Material Inventors Source To FP To NFP Repository Currently Available?
NCRM-1 iPSC Control Episomal Plasmid CD 34+ Cord Blood Mahendra Rao NIH CRM Lonza Contract BMLA MTA Rutgers Yes
NCRM-2 iPSC Control Episomal Plasmid CD 34+ Cord Blood Mahendra Rao NIH CRM Lonza Contract BMLA MTA Rutgers Yes
NCRM-3 iPSC Control Episomal Plasmid CD 34+ Cord Blood Mahendra Rao NIH CRM Lonza Contract BMLA MTA Rutgers Yes
NCRM-4 iPSC Control Episomal Plasmid CD 34+ Cord Blood Mahendra Rao NIH CRM Lonza Contract BMLA MTA Rutgers Yes
NCRM-5 iPSC Control Episomal Plasmid CD 34+ Cord Blood Mahendra Rao NIH CRM Lonza Contract BMLA MTA Rutgers Yes
NCRM-6 iPSC Control Episomal Plasmid CD 34+ Cord Blood Mahendra Rao NIH CRM Lonza Contract BMLA MTA Rutgers Yes
NC1 iPSC Control Retrovirus Fibroblast (female) Manfred Boehm National Heart Lung and Blood Institute BMLA MTA ATCC Yes
NC2 iPSC Control Lentivirus Fibroblast (female) Manfred Boehm National Heart Lung and Blood Institute BMLA MTA ATCC Yes
NC3 iPSC Control Lentivirus HUVECS (male) Manfred Boehm National Heart Lung and Blood Institute BMLA MTA ATCC Yes
NC4 iPSC Control Lentivirus Fibroblast (male) Manfred Boehm National Heart Lung and Blood Institute BMLA MTA ATCC Yes
NC5 iPSC Control Lentivirus Fibroblast (female) Manfred Boehm National Heart Lung and Blood Institute BMLA MTA ATCC Yes
ND1.4 iPSC Control Episomal Plasmid Fibroblast (ATCC) James Thomson University of Wisconsin MTA MTA Rutgers Yes
ND2.0 iPSC Control Episomal Plasmid Fibroblast (ATCC) James Thomson University of Wisconsin MTA MTA Rutgers Yes
BC1 iPSC Control Episomal Plasmid Blood Linzhao Cheng Johns Hopkins University MTA MTA Rutgers Yes
CY2 iPSC Control Episomal Plasmid Blood Cellular Dynamics International (CDI) NIH CRM CDI Contract BLMA MTA Rutgers Yes
REPORTER LINES

These are reporter lines that NIH-CRM will generate. See availability column for lines that have been produced. The table will be updated as other lines become available.

Name or Designation Description iPSC Reprogramming Method Starting Material Inventors Source To FP To NFP Repository Currently Available?
CY2-HB9-Puro-ZsGreen iPSC Reporter - ZsGreen-2A-Puro/PGKp-Hygro at the HB9 genomic site Episomal Plasmid CY2 Mahendra Rao NIH CRM CDI Contract BMLA MTA Rutgers No
CY2-ISL1-Puro-ZsGreen iPSC Reporter - ZsGreen-2A-Puro/PGKp-Hygro at the ISL1 genomic site Episomal Plasmid CY2 Mahendra Rao NIH CRM CDI Contract BMLA MTA Rutgers No
CY2-NES-Puro-ZsGreen iPSC Reporter - ZsGreen-2A-Puro at the NES genomic site Episomal Plasmid CY2 Mahendra Rao NIH CRM CDI Contract BMLA MTA Rutgers No
CY2-NEUROG3-Puro-ZsGreen iPSC Reporter - ZsGreen-2A-Puro/PGKp-Hygro at the NEUROG3 genomic site Episomal Plasmid CY2 Mahendra Rao NIH CRM CDI Contract BMLA MTA Rutgers No
CY2-SOX9-Puro-ZsGreen iPSC Reporter - ZsGreen-2A-Puro/PGKp-Hygro at the SOX9 genomic site Episomal Plasmid CY2 Mahendra Rao NIH CRM CDI Contract BMLA MTA Rutgers No
CY2-DCXp-AAVS1-Puro-ZsGreen iPSC Reporter - DCXp-ZsGreen-2A-Puro/PGKp-Hygro at the AAVS1 safe harbor site Episomal Plasmid CY2 Mahendra Rao NIH CRM CDI Contract BMLA MTA Rutgers No
CY2-EEF1A1p-AAVS1-Puro-ZsGreen iPSC Reporter - EEF1A1p-ZsGreen-2A-Puro at the AAVS1 safe harbor site Episomal Plasmid CY2 Mahendra Rao NIH CRM CDI Contract BMLA MTA Rutgers Yes
CY2-PDX1p-AAVS1-Puro-ZsGreen iPSC Reporter - PDX1p-ZsGreen-2A-Puro/PGKp-Hygro at the AAVS1 safe harbor site Episomal Plasmid CY2 Mahendra Rao NIH CRM CDI Contract BMLA MTA Rutgers Yes
CY2-RPE65p-AAVS1-Puro-ZsGreen iPSC Reporter - RPE65p-ZsGreen-2A-Puro/PGKp-Hygro at the AAVS1 safe harbor site Episomal Plasmid CY2 Mahendra Rao NIH CRM CDI Contract BMLA MTA Rutgers No
CY2-TNNT2p-AAVS1-Puro-ZsGreen iPSC Reporter - TNNT2p-ZsGreen-2A-Puro/PGKp-Hygro at the AAVS1 safe harbor site Episomal Plasmid CY2 Mahendra Rao NIH CRM CDI Contract BMLA MTA Rutgers Yes
DIFFERENTIATED LINES

Differentiated lines will only be available for collaborative projects. Once a line becomes available the table will be updated.

Note: The following cell line names have been changed, as outlined below. Please use the new names when requesting.
1. NSC-BSC1 has been renamed NSC-BC1.
2. NSC-X1 has been renamed NSC-NCRM1.

Name or Designation Description iPSC Reprogramming Method Starting Material Inventors Source To FP To NFP Repository Currently Available?
DN-BC1 Dopaminergic Neuron Episomal Plasmid BC1 Mahendra Rao NIH CRM Lonza Contract BMLA MTA TBA No
DN-X1 Dopaminergic Neuron Episomal Plasmid X1 Mahendra Rao NIH CRM Lonza Contract BMLA MTA TBA No
DN-H14 Dopaminergic Neuron N/A (hESC-H14) H14 Mahendra Rao NIH CRM Lonza Contract BMLA MTA TBA No
MNP-BC1 Motor Neuron Progenitor Episomal Plasmid BC1 Mahendra Rao NIH CRM Lonza Contract BMLA MTA TBA Yes
MNP-X1 Motor Neuron Progenitor Episomal Plasmid X1 Mahendra Rao NIH CRM Lonza Contract BMLA MTA TBA No
MNP-H14 Motor Neuron Progenitor N/A (hESC-H14) H14 Mahendra Rao NIH CRM Lonza Contract BMLA MTA TBA Yes
NSC-BC1 Neural Stem Cell Episomal Plasmid BC1 Mahendra Rao NIH CRM Lonza Contract BMLA MTA TBA Yes
NSC-NCRM1 Neural Stem Cell Episomal Plasmid NCRM-1 Mahendra Rao NIH CRM Lonza Contract BMLA MTA TBA Yes
NSC-H14 Neural Stem Cell N/A (hESC-H14) H14 Mahendra Rao NIH CRM Lonza Contract BMLA MTA TBA Yes

This page was last modified on May 7, 2013